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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX84948 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84948, RRID:AB_10729138
- Product name
- Alpha fetoprotein / AFP antibody [3G3/2D3]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-AFP mouse monoclonal antibody (GTX84948) immunofluorescent staining of COS7 cells transiently transfected with AFP
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody(GTX84948), and then analyzed by flow cytometry.
