Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000174-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000174-M01, RRID:AB_536756
- Product name
- Mouse Monoclonal alpha-Fetoprotein/AFP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AFP - alpha-fetoprotein
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Magnetofluorescent nanocomposites and quantum dots used for optimal application in magnetic fluorescence-linked immunoassay.
Multifunctional nanoparticles for protein detections in thin channels.
A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for the diagnosis of marker proteins.
Immunodevice for simultaneous detection of two relevant tumor markers based on separation of different microparticles by dielectrophoresis.
Tsai HY, Li SY, Fuh CB
Analytical and bioanalytical chemistry 2018 Mar;410(7):1923-1929
Analytical and bioanalytical chemistry 2018 Mar;410(7):1923-1929
Multifunctional nanoparticles for protein detections in thin channels.
Tsai H, Lin W, Chuang M, Lu Y, Fuh CB
Biosensors & bioelectronics 2017 Apr 15;90:153-158
Biosensors & bioelectronics 2017 Apr 15;90:153-158
A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for the diagnosis of marker proteins.
Funano S, Sugahara M, Henares TG, Sueyoshi K, Endo T, Hisamoto H
The Analyst 2015 Mar 7;140(5):1459-65
The Analyst 2015 Mar 7;140(5):1459-65
Immunodevice for simultaneous detection of two relevant tumor markers based on separation of different microparticles by dielectrophoresis.
Ramón-Azcón J, Yasukawa T, Mizutani F
Biosensors & bioelectronics 2011 Oct 15;28(1):443-9
Biosensors & bioelectronics 2011 Oct 15;28(1):443-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - AFP monoclonal antibody (M01), clone 1G7 Analysis of AFP expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of monoclonal antibody to AFP on HepG2 cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AFP MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunoperoxidase of monoclonal antibody to AFP ( Cat # H00000174-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]