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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB13692-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal alpha-Fetoprotein/AFP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of undifferentiated and differentiated IBJ6 human induced pluripotent stem cells, and HepG2 and Hep3B cell lines. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP in the differentiated IBJ6 cells at approximately 74 kDa (as indicated), the HepG2 cells and Hep3B cells. This experiment was conducted under reducing conditions.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Lane view shows a specific band for Fetoprotein-AFP in 0.2 mg/mL of HepG2 and HepB3 lysate. Antibody at 5 ug/mL. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
