Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 710486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AFP Recombinant Polyclonal Antibody (9HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references MYC-driven inhibition of the glutamate-cysteine ligase promotes glutathione depletion in liver cancer.
Anderton B, Camarda R, Balakrishnan S, Balakrishnan A, Kohnz RA, Lim L, Evason KJ, Momcilovic O, Kruttwig K, Huang Q, Xu G, Nomura DK, Goga A
EMBO reports 2017 Apr;18(4):569-585
EMBO reports 2017 Apr;18(4):569-585
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Fetoprotein in whole cell extracts of MCF-7 (lane 1) and A549 cells (lane 2) using an Alpha Fetoprotein Recombinant Rabbit Polyclonal Antibody (Product # 710486) at a dilution of 1 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~68kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Fetoprotein in HeLa cells using an Alpha Fetoprotein Recombinant Rabbit Polyclonal Antibody (Product # 710486) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL