TA501783

antibody from OriGene

Targeting: AFP FETA, HPAFP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501783

Provider

OriGene

Proper citation

OriGene Cat#TA501783, RRID:AB_11130008

Product name

Anti-AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)

Antibody type

Monoclonal

Description

Anti-AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)

Host

Mouse

Conjugate

Unconjugated

Epitope

AFP

Isotype

IgG

Antibody clone number

OTI1E8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501783, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody(TA501783), and then analyzed by flow cytometry.

Validation comment

FC