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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501785 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501785, RRID:AB_11125399
- Product name
- AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
- Antibody type
- Monoclonal
- Description
- AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AFP
- Isotype
- IgG
- Antibody clone number
- OTI1D1
- Vial size
- 100 µl
- Concentration
- 0.64 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AFP mouse monoclonal antibody. (TA501785)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AFP mouse monoclonal antibody. (TA501785)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (TA501785)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-AFP antibody(TA501785),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
