Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501789 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501789, RRID:AB_11125605
- Product name
- AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)
- Antibody type
- Monoclonal
- Description
- AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AFP
- Isotype
- IgG
- Antibody clone number
- OTI3G3
- Vial size
- 100 µl
- Concentration
- 0.31 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AFP mouse monoclonal antibody (TA501789) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody(TA501789), and then analyzed by flow cytometry.
- Validation comment
- FC
