TA502169

antibody from OriGene

Targeting: AFP FETA, HPAFP

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502169

Provider

OriGene

Proper citation

OriGene Cat#TA502169, RRID:AB_2622361

Product name

AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2H7 (formerly 2H7)

Antibody type

Monoclonal

Description

AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2H7 (formerly 2H7)

Host

Mouse

Conjugate

Unconjugated

Epitope

AFP

Isotype

IgG

Antibody clone number

OTI2H7

Vial size

100 µl

Concentration

0.8 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-AFP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody(TA502169), and then analyzed by flow cytometry.

Validation comment

FC