ABIN458119

antibody from antibodies-online

Targeting: AFP FETA, HPAFP

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

ABIN458119

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN458119, RRID:AB_10766387

Product name

anti-alpha-Fetoprotein (AFP) antibody (FITC)

Antibody type

Polyclonal

Antigen

Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum, using a proprietary multiple step method. Several foetoproteins have been demonstrated in human foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64,000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. In pooled foetal/newborn serum a concentration of 1 mg/ml has been measured. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Description

Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Reactivity

Human

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Vial size

1 mL

Concentration

IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.5. No foreign proteins added.

Storage

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.