NBP2-03084

antibody from Novus Biologicals

Targeting: AFP FETA, HPAFP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03084

Provider

Novus Biologicals

Product name

Mouse Monoclonal alpha-Fetoprotein/AFP Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha 1 Fetoprotein.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.