Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-11480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AFP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Establishment and characterization of a novel cell line (cc‑006cpm8) of moderately/poorly differentiated colorectal adenocarcinoma derived from a primary tumor of a patient.
Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP.
Chu X, Xue Y, Huo X, Wei J, Chen Y, Han R, Chen H, Su X, Zhang H, Gong Y, Chen J
International journal of oncology 2019 Jul;55(1):243-256
International journal of oncology 2019 Jul;55(1):243-256
Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP.
Zhang W, Lu Z, Kong G, Gao Y, Wang T, Wang Q, Cai N, Wang H, Liu F, Ye L, Zhang X
Molecular cancer 2014 May 28;13:128
Molecular cancer 2014 May 28;13:128
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an AFP polyclonal antibody (Product # PA5-11480) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using an AFP polyclonal antibody (Product # PA5-11480), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 HBx accelerates hepatocarcinogenesis with partner survivin. (A) The expression of survivin in the liver tissues of p21-HBx Tg mice aged 6, 12 and 18 mouths versus WT littermate mice were determined by western blotting, respectively (** P < 0.01, Student's t test). (B) The integrations of HBx and survivin genes into the genomes of LO2 cells were validated by PCR using genomic DNA as a template. GAPDH was used as a loading control. (C) The effect of HBx and/or survivin on cell proliferation was detected by colony-formation assay (* P < 0.05, ** P < 0.01, Student's t test). (D) Tumor formation in nude mice (n = 8 per group) injected with LO2-X or LO2-X-S cells was assessed in 3 weeks. The expression of AFP was tested in the tumor tissues from mice by western blotting and IHC analysis, respectively.