PA5-120663

antibody from Invitrogen Antibodies

Targeting: RAD21 hHR21, KIAA0078, SCC1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120663

Provider

Invitrogen Antibodies

Product name

RAD21 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Jurkat, Mouse thymus, C6. Immunogen sequence: VDPVEPMPTM TDQTTLVPNE EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL MMWKETGGVE KLFSLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.96 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RAD21 in U2OS cells. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RAD21 in 300 μg extracts of Jurkat cells. Samples were precipitated with 3 μg RAD21 Polyclonal antibody (Product # PA5-120663). Western blot was performed from the immunoprecipitate using RAD21 Polyclonal antibody (Product # PA5-120663) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RAD21 in 300 μg extracts of Jurkat cells. Samples were precipitated with 3 μg RAD21 Polyclonal antibody (Product # PA5-120663). Western blot was performed from the immunoprecipitate using RAD21 Polyclonal antibody (Product # PA5-120663) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAD21 in paraffin-embedded human colon carcinoma. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAD21 in paraffin-embedded mouse stomach. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAD21 in paraffin-embedded rat stomach. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of RAD21 in extracts of A-549 cells using a RAD21 Polyclonal antibody (Product # PA5-120663) and rabbit amount of immunoprecipitated DNA was checked by quantitative PCR. A histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of RAD21 in extracts of A-549 cells. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of RAD21 in extracts of A-549 cells. Samples were incubated with RAD21 Polyclonal antibody (Product # PA5-120663) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.