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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB100-1465 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1465, RRID:AB_2154903
- Product name
- Goat Polyclonal LXR alpha/beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Clinical relevance of cholesterol homeostasis genes in colorectal cancer.
Liver X receptor agonist inhibits proliferation of ovarian carcinoma cells stimulated by oxidized low density lipoprotein.
Identification of macrophage liver X receptors as inhibitors of atherosclerosis.
Sharma B, Gupta V, Dahiya D, Kumar H, Vaiphei K, Agnihotri N
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Oct;1864(10):1314-1327
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Oct;1864(10):1314-1327
Liver X receptor agonist inhibits proliferation of ovarian carcinoma cells stimulated by oxidized low density lipoprotein.
Scoles DR, Xu X, Wang H, Tran H, Taylor-Harding B, Li A, Karlan BY
Gynecologic oncology 2010 Jan;116(1):109-16
Gynecologic oncology 2010 Jan;116(1):109-16
Identification of macrophage liver X receptors as inhibitors of atherosclerosis.
Tangirala RK, Bischoff ED, Joseph SB, Wagner BL, Walczak R, Laffitte BA, Daige CL, Thomas D, Heyman RA, Mangelsdorf DJ, Wang X, Lusis AJ, Tontonoz P, Schulman IG
Proceedings of the National Academy of Sciences of the United States of America 2002 Sep 3;99(18):11896-901
Proceedings of the National Academy of Sciences of the United States of America 2002 Sep 3;99(18):11896-901
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LXR alpha/beta Antibody [NB100-1465] - (1ug/ml) Analysis of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LXR alpha/beta Antibody [NB100-1465] - Staining of Human Liver (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LXR alpha/beta Antibody [NB100-1465] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
