Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-24365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF174 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Applications Tested: This eBioSN4 (SN4 C3-3A2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of ZNF174 was performed by loading Jurkat and HepG2 cells. Proteins were transferred to a membrane and probed with a ZNF174 Polyclonal Antibody (Product # PA1-24365). Lane A : Jurkat cell lysate ( primary antibody used : 5.0 µg/mL), Lane B : HepG2 cell lysate (primary antibody used : 2.5 µg/mL)