H00007727-M01

antibody from Abnova Corporation

Targeting: ZNF174 ZSCAN8

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007727-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007727-M01, RRID:AB_425747

Product name

ZNF174 monoclonal antibody (M01), clone 2D7-E9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ZNF174.

Antigen sequence

MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPL
QKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQL
CRQWLQPELHTKEQILELLVMEQFLTILPPEIQAR
VRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQ
GQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSP
AEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTEL
LIEKTDPNMATDELPCKLWLSFIA


Isotype

IgG

Antibody clone number

2D7-E9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ZNF174 monoclonal antibody (M01), clone 2D7-E9 Western Blot analysis of ZNF174 expression in SW-13 ( Cat # L005V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ZNF174 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to ZNF174 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol