A302-216A

antibody from Invitrogen Antibodies

Targeting: DDX27 dJ686N3.1, DRS1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-216A

Provider

Invitrogen Antibodies

Product name

DDX27 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse DDX27 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DDX27 (Cat. No. A302-216A) used at a dilution of 1:5,000 ( 0.2µg/ml) and 1:1,000 ( 1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DDX27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX27 antibody A302-216A used for IP at 10 µg/mg lysate. DDX27 was also immunoprecipitated by rabbit anti-DDX27 antibody A302-217A, which recognizes a downstream epitope. For blotting immunoprecipitated DDX27, A302-217A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.