NB100-56505

antibody from Novus Biologicals

Targeting: TNFRSF11B OCIF, OPG, TR1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-56505

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-56505, RRID:AB_838658

Product name

Mouse Monoclonal Osteoprotegerin/TNFRSF11B Antibody

Antibody type

Monoclonal

Description

Protein G purified. The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).

Reactivity

Human, Mouse, Rat, Rabbit

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1α, RANKL and OPG.

Bayer Alinca S, Sağlam E, Zengin Celik T, Hacisalihoglu P, Doğan MA
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2020 Apr 15;:1-8

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Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction

Wang X, Cao Y, Liu Z, Wang Z, Chu X, Wang L, Hu X, Zhao H, Diao Z, Peng F, Ye H, Cao Z
Balkan medical journal 2019 Oct 28;36(6):311-319

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Comparing the Osteogenic Potentials and Bone Regeneration Capacities of Bone Marrow and Dental Pulp Mesenchymal Stem Cells in a Rabbit Calvarial Bone Defect Model.

Lee YC, Chan YH, Hsieh SC, Lew WZ, Feng SW
International journal of molecular sciences 2019 Oct 10;20(20)

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Role of interleukin-33 in the clinical pathogenesis of chronic apical periodontitis.

Gegen T, Zhu Y, Sun Q, Hou B
The Journal of international medical research 2019 Jul;47(7):3332-3343

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Resolvin E1 Promotes Bone Preservation Under Inflammatory Conditions.

El Kholy K, Freire M, Chen T, Van Dyke TE
Frontiers in immunology 2018;9:1300

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Osteoblastic heparan sulfate regulates osteoprotegerin function and bone mass.

Nozawa S, Inubushi T, Irie F, Takigami I, Matsumoto K, Shimizu K, Akiyama H, Yamaguchi Y
JCI insight 2018 Feb 8;3(3)

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Nano-sized Al₂O₃ particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts.

Li, Wang C, Li Z, Wang H, He J, Zhu J, Zhang Y, Shen C, Xiao F, Gao Y, Zhang X, Li Y, Wang P, Peng J, Cai G, Zuo B, Yang Y, Shen Y, Song W, Zhang X, Shen L, Chen X
Cell death & disease 2018 Aug 6;9(8):840

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Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis.

Scimeca M, Salustri A, Bonanno E, Nardozi D, Rao C, Piccirilli E, Feola M, Tancredi V, Rinaldi A, Iolascon G, Orlandi A, Gasbarra E, Maffulli N, Brandi ML, Tarantino U
Cell death & disease 2017 Oct 12;8(10):e3125

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Influence of receptor activator of nuclear factor kappa B ligand, osteoprotegerin and interleukin-33 on bone metabolism in patients with long-standing ulcerative colitis.

Stanisławowski M, Wiśniewski P, Guzek M, Wierzbicki PM, Adrych K, Smoczyński M, Sworczak K, Celiński K, Kmieć Z
Journal of Crohn's & colitis 2014 Aug;8(8):802-10

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Characterization of osteoblastic and osteolytic proteins in prostate cancer bone metastases.

Larson SR, Zhang X, Dumpit R, Coleman I, Lakely B, Roudier M, Higano CS, True LD, Lange PH, Montgomery B, Corey E, Nelson PS, Vessella RL, Morrissey C
The Prostate 2013 Jun;73(9):932-40

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Rituximab abrogates joint destruction in rheumatoid arthritis by inhibiting osteoclastogenesis.

Boumans MJ, Thurlings RM, Yeo L, Scheel-Toellner D, Vos K, Gerlag DM, Tak PP
Annals of the rheumatic diseases 2012 Jan;71(1):108-13

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Expression of osteoprotegerin in human fat tissue; implications for chronic kidney disease.

Witasp A, Carrero JJ, Hammarqvist F, Qureshi AR, Heimbürger O, Schalling M, Lindholm B, Nordfors L, Stenvinkel P
European journal of clinical investigation 2011 May;41(5):498-506

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Cutaneous HPV5 E6 causes increased expression of Osteoprotegerin and Interleukin 6 which contribute to evasion of UV-induced apoptosis.

Tomlins C, Storey A
Carcinogenesis 2010 Dec;31(12):2155-64

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Noncollagenous bone matrix proteins as a part of calcific aortic valve disease regulation.

Pohjolainen V, Taskinen P, Soini Y, Rysä J, Ilves M, Juvonen T, Ruskoaho H, Leskinen H, Satta J
Human pathology 2008 Nov;39(11):1695-701

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The abundant synovial expression of the RANK/RANKL/Osteoprotegerin system in peripheral spondylarthritis is partially disconnected from inflammation.

Vandooren B, Cantaert T, Noordenbos T, Tak PP, Baeten D
Arthritis and rheumatism 2008 Mar;58(3):718-29

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The antibody MAB8051 directed against osteoprotegerin detects carbonic anhydrase II: implications for association studies with human cancers.

Waterman EA, Cross NA, Lippitt JM, Cross SS, Rehman I, Holen I, Hamdy FC, Eaton CL
International journal of cancer 2007 Nov 1;121(9):1958-66

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RANKL protein is expressed at the pannus-bone interface at sites of articular bone erosion in rheumatoid arthritis.

Pettit AR, Walsh NC, Manning C, Goldring SR, Gravallese EM
Rheumatology (Oxford, England) 2006 Sep;45(9):1068-76

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Osteoprotegerin (OPG) binds with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): suppression of TRAIL-induced apoptosis in ameloblastomas.

Sandra F, Hendarmin L, Nakamura S
Oral oncology 2006 Apr;42(4):415-20

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Hepatic osteodystrophy: does the osteoprotegerin/receptor activator of nuclear factor-kB ligand system play a role?

Gaudio A, Lasco A, Morabito N, Atteritano M, Vergara C, Catalano A, Fries W, Trifiletti A, Frisina N
Journal of endocrinological investigation 2005 Sep;28(8):677-82

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Hepatic osteodystrophy: does the osteoprotegerin/receptor activator of nuclear factor-kB ligand system play a role?

Gaudio A, Lasco A, Morabito N, Atteritano M, Vergara C, Catalano A, Fries W, Trifiletti A, Frisina N
Journal of endocrinological investigation 2005 Sep;28(8):677-82

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Osteoprotegerin is expressed in colon carcinoma cells.

Pettersen I, Bakkelund W, Smedsrød B, Sveinbjørnsson B
Anticancer research 2005 Nov-Dec;25(6B):3809-16

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Association of osteoprotegerin with human abdominal aortic aneurysm progression.

Moran CS, McCann M, Karan M, Norman P, Ketheesan N, Golledge J
Circulation 2005 Jun 14;111(23):3119-25

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Ameloblastoma induces osteoclastogenesis: a possible role of ameloblastoma in expanding in the bone.

Sandra F, Hendarmin L, Kukita T, Nakao Y, Nakamura N, Nakamura S
Oral oncology 2005 Jul;41(6):637-44

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The RANKL/OPG system and bone mineral density in patients with chronic liver disease.

Moschen AR, Kaser A, Stadlmann S, Millonig G, Kaser S, Mühllechner P, Habior A, Graziadei I, Vogel W, Tilg H
Journal of hepatology 2005 Dec;43(6):973-83

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The RANKL/OPG system is activated in inflammatory bowel disease and relates to the state of bone loss.

Moschen AR, Kaser A, Enrich B, Ludwiczek O, Gabriel M, Obrist P, Wolf AM, Tilg H
Gut 2005 Apr;54(4):479-87

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Osteoprotegerin production by human intestinal epithelial cells: a potential regulator of mucosal immune responses.

Vidal K, Serrant P, Schlosser B, van den Broek P, Lorget F, Donnet-Hughes A
American journal of physiology. Gastrointestinal and liver physiology 2004 Oct;287(4):G836-44

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Osteoprotegerin in human milk: a potential role in the regulation of bone metabolism and immune development.

Vidal K, van den Broek P, Lorget F, Donnet-Hughes A
Pediatric research 2004 Jun;55(6):1001-8

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Osteoprotegerin and osteopontin are expressed at high concentrations within symptomatic carotid atherosclerosis.

Golledge J, McCann M, Mangan S, Lam A, Karan M
Stroke 2004 Jul;35(7):1636-41

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Osteoprotegerin is bound, internalized, and degraded by multiple myeloma cells.

Standal T, Seidel C, Hjertner Ø, Plesner T, Sanderson RD, Waage A, Borset M, Sundan A
Blood 2002 Oct 15;100(8):3002-7

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Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression.

Pearse RN, Sordillo EM, Yaccoby S, Wong BR, Liau DF, Colman N, Michaeli J, Epstein J, Choi Y
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11581-6

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Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.

Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90

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Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.

Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90

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17beta-estradiol stimulates expression of osteoprotegerin by a mouse stromal cell line, ST-2, via estrogen receptor-alpha.

Saika M, Inoue D, Kido S, Matsumoto T
Endocrinology 2001 Jun;142(6):2205-12

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The osteoprotegerin/receptor activator of nuclear factor kappaB/receptor activator of nuclear factor kappaB ligand system in cartilage.

Komuro H, Olee T, Kühn K, Quach J, Brinson DC, Shikhman A, Valbracht J, Creighton-Achermann L, Lotz M
Arthritis and rheumatism 2001 Dec;44(12):2768-76

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The osteoprotegerin/receptor activator of nuclear factor kappaB/receptor activator of nuclear factor kappaB ligand system in cartilage.

Komuro H, Olee T, Kühn K, Quach J, Brinson DC, Shikhman A, Valbracht J, Creighton-Achermann L, Lotz M
Arthritis and rheumatism 2001 Dec;44(12):2768-76

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Force-induced osteoclast apoptosis in vivo is accompanied by elevation in transforming growth factor beta and osteoprotegerin expression.

Kobayashi Y, Hashimoto F, Miyamoto H, Kanaoka K, Miyazaki-Kawashita Y, Nakashima T, Shibata M, Kobayashi K, Kato Y, Sakai H
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2000 Oct;15(10):1924-34

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Staining of human liver. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Staining of human heart tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin antibody generated a diffused but weak cytoplasmic staining with a very specific and strong punctate signal at the periphery of the cells.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary.