A00863

antibody from Boster Biological Technology

Targeting: TNFRSF11B OCIF, OPG, TR1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00863

Provider

Boster Biological Technology

Product name

Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Osteoprotegerin/TNFRSF11B detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Arl13b promotes the proliferation, migration, osteogenesis, and mechanosensation of osteoblasts.

Lin T, Sun Y
Tissue & cell 2023 Jun;82:102088

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Down-regulating of MFN2 promotes vascular calcification via regulating RAS-RAF-ERK1/2 pathway.

Zhang WB, Feng SY, Xiao ZX, Qi YF, Zeng ZF, Chen H
International journal of cardiology 2022 Nov 1;366:11-18

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MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p.

Yang X, Zhang Y, Li Y, Wen T
International journal of molecular medicine 2018 Mar;41(3):1536-1546

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Anti-osteoporotic activity of puerarin 6"-O-xyloside on ovariectomized mice and its potential mechanism.

Li H, Chen B, Pang G, Chen J, Xie J, Huang H
Pharmaceutical biology 2016;54(1):111-7

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Alendronate-loaded hydroxyapatite-TiO(2) nanotubes for improved bone formation in osteoporotic rabbits.

Shen X, Ma P, Hu Y, Xu G, Xu K, Chen W, Ran Q, Dai L, Yu Y, Mu C, Cai K
Journal of materials chemistry. B 2016 Feb 28;4(8):1423-1436

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Calcitonin gene-related peptide inhibits osteolytic factors induced by osteoblast in co-culture system with breast cancer.

Zhao H, Ning LL, Wang ZY, Li HT, Qiao D, Yao Y, Qin HL
Cell biochemistry and biophysics 2014 Nov;70(2):1097-104

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Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus.

Zhao H, Li X, Li N, Liu T, Liu J, Li Z, Xiao H, Li J
The British journal of nutrition 2014 Mar 14;111(5):836-46

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Porphyromonas endodontalis lipopolysaccharides induce RANKL by mouse osteoblast in a way different from that of Escherichia coli lipopolysaccharide.

Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X
Journal of endodontics 2011 Dec;37(12):1653-8

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). TNFRSF11B was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image