500-P149-100UG

antibody from Invitrogen Antibodies

Targeting: TNFRSF11B OCIF, OPG, TR1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P149-100UG

Provider

Invitrogen Antibodies

Product name

TNFRSF11B Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Bisphosphonates Reduce Smoking-Induced Osteoporotic-Like Alterations by Regulating RANKL/OPG in an Osteoblast and Osteoclast Co-Culture Model.

Zhu S, Häussling V, Aspera-Werz RH, Chen T, Braun B, Weng W, Histing T, Nussler AK
International journal of molecular sciences 2020 Dec 23;22(1)

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A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension.

Arnold ND, Pickworth JA, West LE, Dawson S, Carvalho JA, Casbolt H, Braithwaite AT, Iremonger J, Renshall L, Germaschewski V, McCourt M, Bland-Ward P, Kowash H, Hameed AG, Rothman AMK, Frid MG, Roger Thompson AA, Evans HR, Southwood M, Morrell NW, Crossman DC, Whyte MKB, Stenmark KR, Newman CM, Kiely DG, Francis SE, Lawrie A
Nature communications 2019 Nov 15;10(1):5183

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Role of Wnt/β-catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients.

Folestad A, Ålund M, Asteberg S, Fowelin J, Aurell Y, Göthlin J, Cassuto J
Acta orthopaedica 2015;86(4):415-25

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Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models.

Liu Y, Hawkins OE, Vilgelm AE, Pawlikowski JS, Ecsedy JA, Sosman JA, Kelley MC, Richmond A
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Dec 1;21(23):5338-48

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human OPG by Western Blot analysis TNFRSF11B Polyclonal Antibody (Product # 500-P149-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human OPG by Western Blot analysis TNFRSF11B Polyclonal Antibody (Product # 500-P149-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human OPG by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TNFRSF11B Polyclonal Antibody (Product # 500-P149-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TNFRSF11B Polyclonal Antibody, Biotin (Product # 500-P149BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human OPG.