34-6200

antibody from Invitrogen Antibodies

Targeting: MAG GMA, S-MAG, SIGLEC-4A, SIGLEC4A

Provider product page for 34-6200

Western blot

Immunoprecipitation

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [19]
Comments [0]
Validations
Other assay [8]

Submit

Product number: 34-6200 - Provider product page
Provider: Invitrogen Antibodies
Product name: MAG Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 0.25 mg/mL
Storage: -20°C

MAG protein structure - 34-6200 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Administration of an NOR antagonist results in sex-specific acceleration of rat brain myelination. (a) Experimental schedule of BAN-ORL 24 administration: 9-day-old female and male pups were administered the blood brain barrier permeable NOR antagonist BAN-ORL24 (1 mg/kg/day, IP) or vehicle (controls). Animals were sacrificed at postnatal day 14, and the cerebral hemispheres analyzed for the expression of MBP, MAG and MOG, using beta-Actin levels as loading controls. The figure shows representative western blots for the female (b-d) and male (e-g) pups. Bar graphs are the mean +- SEM from a total of at least 9 pups/group/sex from three different litters. **** p < .001, ** p < .006

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Representative Western immunoblotting for P0 and MAG protein in compression phase ( a ) and decompression phase ( b ) (P0, myelin protein 0; MAG, myelin associated glycoprotein; Sham, nerves of sham-operated mice; PBS, nerves of untreated mice during compression phase; CF, nerves of mice treated with clemastine during compression phase; d-PBS, nerves of untreated mice after surgical decompression; d-CF, nerves of mice treated with clemastine after surgical decompression). Full-length Western blots are presented in Supplementary Figs. 1 and 2 . Band intensity quantification of P0 and MAG of CF group as fold change of PBS group. Band intensity quantification of P0 and MAG of d-CF group as fold change of d-PBS group. ss-actin was used to normalize the intensity of the bands. Error bars represent standard error of mean. Mann-Whitney U test was conducted (*p < 0.05, and n = 3 or 4 animals per group).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Analyses of the myelin compartment in treated SD mice (A) Quantification of myelinated fibers and percentage of demyelinated/degenerated (aberrant) fibers among total fibers in SC sections from treated mice (IC GT, BMT, and IC GT + BMT) at 120 days and 240 days and age-matched UT controls (WT and SD). Data are expressed as mean +- SEM; n = 3-4 mice/group; nonparametric one-way ANOVA followed by Dunn's multiple comparisons test, *p < 0.05. (B) Semithin section analysis of lumbar SC of IC GT + BMT-treated SD mice at 120 days and 240 days and UT controls (WT and SD). Red arrows indicate aberrant myelin. Scale bar, 10 mum. (C) Electron microscopy images of optic nerves in treated SD and UT controls at 120 days and 240 days. Red arrows indicate aberrant myelin, and asterisks mark macrophages. Scale bar, 2 mum. (D) Representative western blot analyses and relative quantification showing MBP and MAG protein expression in whole-brain lysate (TEL and CB) of treated mice (IC GT, BMT, and IC GT + BMT) at 120 days and 240 days and age-matched UT controls (WT, Het, and SD). Data are expressed as fold change with respect to the WT (set as 1) after normalization to beta-actin expression. Data represent the mean +- SEM; n = 3-10 mice/group. One-way ANOVA followed by Kruskal-Wallis multiple comparisons test, *p < 0.05. (E) Expression of myelin-related genes ( Mbp , Mag , Plp1 , and Ugt8a ) in whole-brain lysate (TEL and CB) of treated mice (IC GT, BMT, and IC GT + BMT) at 120 days and 240 day

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Etv1 expression and cellular localization in the crushed mouse sciatic nerve. (A) WB analysis showing the expression of Etv1 and MAG in nerves on days 1, 4, 7, 14, 21, and 28 after nerve injury. The uninjured nerve served as the control (Ctrl). Quantitative statistical comparisons of Etv1 and MAG protein levels in regenerated nerve segments after nerve crush are shown in the histograms. ***, P < 0.001, one-way ANOVA, n=3 per group. (B) Immunofluorescence staining of the injured sciatic nerve sections of mice show only a small amount of Etv1 and S100beta co-localization on day 1 post-injury. Etv1 is mainly localized in SCs from day 4 after injury. Scale bar =100 mum. S100beta (a marker of SCs) is stained green and Etv1 is stained red. MAG, myelin-associated glycoprotein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SC, Schwann cell; WB, Western blotting; ANOVA, analysis of variance.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Etv1 knockdown in Schwann cells antagonizes its differentiation. (A) (a1) Representative images of phase contrast microscopy and ICC analysis, showing that dB-cAMP can effectively induce SC differentiation. Red staining represents MAG expression and cell nuclei (blue) are marked with Hoechst staining. Scale bar =100 mum. (a2) WB comparing the expression of MAG in SCs treated with dB-cAMP or vehicle for 24, 48, and 72 hours. ***, P < 0.001; one-way ANOVA; n=3 per group. (B) (b1) Western blot comparing Etv1 levels in SCs differentiated for 24, 48, and 72 hours. ***, P < 0.001; one-way ANOVA; n=3 per group. (b2) WB showing Etv1 and MAG expression in differentiated SCs transfected with Etv1-siRNA and scrambled siRNA. The histogram shows that the knockdown of Etv1 in SCs significantly reduced the expression of MAG after the induction of differentiation. Student's t -test; **, P < 0.01 vs. scramble; n=3 per group. MAG, myelin-associated glycoprotein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Ctrl, control; Dif, differentiation; siRNA, small interfering RNA; ICC, immunocytochemistry; SC, Schwann cell; WB, Western blotting; ANOVA, analysis of variance.