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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunohistochemistry [2]
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- Product number
- PA5-30087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human brain, mouse brain, rat brain. Predicted reactivity: Mouse (95%), Rat (95%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAG using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MAG polyclonal antibody (Product # PA5-30087) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAG using 50 µg rat brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MAG polyclonal antibody (Product # PA5-30087) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAG using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MAG polyclonal antibody (Product # PA5-30087) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAG was performed by separating 30 µg of Human tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAG Polyclonal Antibody (Product # PA5-30087) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAG was performed by separating 50 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAG Polyclonal Antibody (Product # PA5-30087) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MAG Polyclonal Antibody (Product # PA5-30087) and a 100kDa band corresponding to Myelin-associated glycoprotein was observed across was observed across brain and cerebellum tissues but not in stomach, large intestine and skeletal muscle. which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Stomach (Lane 5), Mouse Large Intestine (Lane 6), Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of MAG was performed in frozen-sectioned adult mouse cerebellum tissue using MAG Polyclonal Antibody (Product # PA5-30087) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAG Polyclonal Antibody detects MAG protein at on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAG Polyclonal Antibody (Product # PA5-30087) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
