MA5-26999

antibody from Invitrogen Antibodies

Targeting: MST1 D3F15S2, DNF15S2, HGFL, MSP, NF15S2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MA5-26999

Provider

Invitrogen Antibodies

Product name

MSP Monoclonal Antibody (OTI1A10)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1A10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MST1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with MST1 Monoclonal Antibody (OTI1A10) (Product # MA5-26999) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane and cytoplasm localization. Panel e represents U-87 MG cells showing no expression of MST1. Panel f represents control Hep G2 cells with no primary antibody to assess background. The images were captured at 60X magnification.