PA5-12982

antibody from Invitrogen Antibodies

Targeting: GIT1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-12982

Provider

Invitrogen Antibodies

Product name

Phospho-GIT1 (Tyr554) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of phosphorylated GIT1 was performed by loading human umbilical vein endothelial cell lysates with and without shear stress (at 10 dyn/cm^2) per well onto an SDS PAGE gel. Proteins were transferred to a membrane and blocked with SuperBlock T20 (TBS) Blocking Buffer. The membrane was probed with a Phospho-GIT1 pTyr554 Antibody (Product # PA5-12982) at a dilution of 1:200, washed, and probed with a HRP-conjugated secondary antibody for chemiluminescent detection. β-actin detection (lower panel) was used as loading control. Data courtesy of the Innovators Program.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot blot analysis of Phospho-GIT1 pTyr554 polyclonal antibody (Product # PA5-12982) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot blot analysis of Phospho-GIT1 pTyr554 polyclonal antibody (Product # PA5-12982) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.