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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B6407 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ CCN3 / NOV Antibody (clone 2G8) LS-B6407
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G8
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of NOV expression in transfected 293T cell line by NOV monoclonal antibody, clone 2G8.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of NOV transfected lysate using anti-NOV monoclonal antibody and Protein A magnetic bead, immunoblotted with NOV rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of NOV transfected lysate using anti-NOV monoclonal antibody and Protein A magnetic bead, immunoblotted with NOV rabbit polyclonal antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NOV / CCN3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
