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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1640 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal NOV/CCN3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.
Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney.
Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
Kolb AD, Shupp AB, Mukhopadhyay D, Marini FC, Bussard KM
Breast cancer research : BCR 2019 Feb 27;21(1):31
Breast cancer research : BCR 2019 Feb 27;21(1):31
Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney.
Marti HP, Jeffs A, Scherer A, Leader J, Leader C, Bedford J, Walker R
PloS one 2016;11(12):e0168240
PloS one 2016;11(12):e0168240
Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
Sin WC, Tse M, Planque N, Perbal B, Lampe PD, Naus CC
The Journal of biological chemistry 2009 Oct 23;284(43):29935-44
The Journal of biological chemistry 2009 Oct 23;284(43):29935-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- NOV/CCN3 in Human Kidney Cancer Tissue. NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
