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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1640 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NOV/CCN3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P48745- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney.
Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
Marti HP, Jeffs A, Scherer A, Leader J, Leader C, Bedford J, Walker R
PloS one 2016;11(12):e0168240
PloS one 2016;11(12):e0168240
Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
Sin WC, Tse M, Planque N, Perbal B, Lampe PD, Naus CC
The Journal of biological chemistry 2009 Oct 23;284(43):29935-44
The Journal of biological chemistry 2009 Oct 23;284(43):29935-44
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- NOV/CCN3 in Human Kidney Cancer Tissue. NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
