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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Flow cytometry [1]
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- Product number
- AP2854d - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2854d, RRID:AB_10549202
- Product name
- ENPP2 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center)(Cat. #AP2854d). AP2854d was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center) (Cat. #AP2854d). 293T cell lysates (ÿug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of ENPP2 (arrow) using rabbit polyclonal ENPP2 Antibody (Center) (Cat. #AP2854d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ENPP2 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of ENPP2 Antibody (Center) (Cat. #AP2854d) in mouse cerebellum tissue lysates (35ug/lane). ENPP2 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- ENPP2 Antibody (Center) (Cat. #AP2854d) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
