Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85221 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENPP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.13 mg/mL
Submitted references Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model.
Song Z, Xu Y, Zhang L, Zhou L, Zhang Y, Han Y, Li X, Yu P, Qu Y, Zhao W, Qin C
Frontiers in genetics 2020;11:565479
Frontiers in genetics 2020;11:565479
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENPP2 in A) U87-MG whole cell lysate using ENPP2 polyclonal antibody (Product # PA5-85221) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENPP2 in various tissue extracts using ENPP2 polyclonal antibody (Product # PA5-85221) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ENPP2 was performed by separating 30 µg of Human Cerebrospinal Fluid by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ENPP2 Polyclonal Antibody (Product # PA5-85221) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ENPP2 in 4% paraformaldehyde-fixed SK-N-SH cells using ENPP2 polyclonal antibody (Product # PA5-85221) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Western blots (WB) and quantitative analysis of 11 differential proteins in the urinary exosomes isolated from control and 5XFAD mice. (A) Same aliquot of urinary exosomes isolated from three control samples and three 5XFAD mice samples. AOAH, Clusterin, Ceruloplasmin, Cathepsin B, ENPP2, FUCA2, HEXB, Ly86, Pcolce, Preol, and PrP proteins were detected by WB in sequence. Representative blots of the tested proteins and the molecular weight of standard proteins were presented. (B-L) Quantitative analysis of each of the tested protein's relative fold change, respectively. All data are presented as mean +- SD in triplicate experiments; Student's t -test; * P < 0.05; ** P < 0.01.
