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- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PAB8520 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8520, RRID:AB_1674965
- Product name
- Enpp2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Enpp2.
- Antigen sequence
LKTYLHTYESEI- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The N-terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide.
NPP-type ectophosphodiesterases: unity in diversity.
Identification of autotaxin as a neurite retraction-inducing factor of PC12 cells in cerebrospinal fluid and its possible sources.
Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma.
Koike S, Keino-Masu K, Ohto T, Masu M
Genes to cells : devoted to molecular & cellular mechanisms 2006 Feb;11(2):133-42
Genes to cells : devoted to molecular & cellular mechanisms 2006 Feb;11(2):133-42
NPP-type ectophosphodiesterases: unity in diversity.
Stefan C, Jansen S, Bollen M
Trends in biochemical sciences 2005 Oct;30(10):542-50
Trends in biochemical sciences 2005 Oct;30(10):542-50
Identification of autotaxin as a neurite retraction-inducing factor of PC12 cells in cerebrospinal fluid and its possible sources.
Sato K, Malchinkhuu E, Muraki T, Ishikawa K, Hayashi K, Tosaka M, Mochiduki A, Inoue K, Tomura H, Mogi C, Nochi H, Tamoto K, Okajima F
Journal of neurochemistry 2005 Feb;92(4):904-14
Journal of neurochemistry 2005 Feb;92(4):904-14
Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma.
Tanaka M, Kishi Y, Takanezawa Y, Kakehi Y, Aoki J, Arai H
FEBS letters 2004 Jul 30;571(1-3):197-204
FEBS letters 2004 Jul 30;571(1-3):197-204
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Enpp2 from zebrafish embryo lysate by Enpp2 polyclonal antibody ( Cat # PAB8520 ) (1:500, 4°C, overnight). Instead of a predicted 93.6 KDa band, we detected a ~50KDa single band from shield stage of wild type embryos (lane 1) on a 12% SDS-PAGE, and this band can be efficiently knockdown by Enpp2 morpholino oligonucleotides (land 2 and 3). This phenomenon is probably due to a cleavage of endogenous Enpp2 under reducing condition of SDS-PAGE as previously reported in human and mice. (Journal of Biochemistry, 283(12):7776-7789)(By courtesy of Shih-Lei Lai1 and Shyh-Jye Lee1-4; 1Institute of Zoology, 2Department of Life Science, 3Center for Biotechnology, 4Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of mouse spinal cord, using Enpp2 polyclonal antibody (Cat # PAB8520) .
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of mouse thalamus, using Enpp2 polyclonal antibody (Cat # PAB8520) .
- Validation comment
- Immunofluorescence
