- PA5-12478 - Invitrogen Antibodies ENPP2 antibody

Submitted references Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.

Rai NK, Singh V, Li L, Willard B, Tripathi A, Dutta R
Frontiers in neurology 2021;12:779003

Interaction of Autotaxin With Lipoprotein(a) in Patients With Calcific Aortic Valve Stenosis.

Bourgeois R, Devillers R, Perrot N, Després AA, Boulanger MC, Mitchell PL, Guertin J, Couture P, Boffa MB, Scipione CA, Pibarot P, Koschinsky ML, Mathieu P, Arsenault BJ
JACC. Basic to translational science 2020 Sep;5(9):888-897

Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.

Sikura KÉ, Potor L, Szerafin T, Oros M, Nagy P, Méhes G, Hendrik Z, Zarjou A, Agarwal A, Posta N, Torregrossa R, Whiteman M, Fürtös I, Balla G, Balla J
British journal of pharmacology 2020 Feb;177(4):793-809

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using an ENPP2 polyclonal antibody (Product # PA5-12478) in Y79 cell lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human hepatocarcinoma using an ENPP2 polyclonal antibody (Product # PA5-12478), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of MDA-MB435 cells using an ENPP2 polyclonal antibody (Product # PA5-12478) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 AP72 enhances generation of PPi. VIC were cultured in growth medium or calcification medium alone or supplemented with AP72 (20 mumol*L -1 ) for 5 days. Differences in (a) ENPP2 protein and mRNA levels, (b) ANK1 protein and mRNA levels, (c) pyrophosphate level measured using a PPiLight pyrophosphate detection kit are presented. (d) Representative ENPP2 western blot from AI and AS tissue lysate of heart valves. (e) Pyrophosphate levels of heart valve tissues were measured using a pyrophosphate detection kit. Data shown are means +- SEM of five independent experiments. * P < .05, significantly different as indicated; ns, not significant

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 MS brain proteome analysis and validation in WMLs. (A) Volcano plot displaying the distribution of all identified proteins ( n = 786) with relative protein abundance (log2 fold change) plotted against levels of significance (-Log 10 p -value). 105 proteins were significantly upregulated (red circle) while 119 were downregulated (blue circle) in WMLs. (B) Mean LFQ intensity-based heatmap showing the top 40 dysregulated proteins (20 upregulated and 20 downregulated) in MS WMLs. The color range of LFQ intensity extends from low (dark blue) to high (dark red). (C) IPA canonical pathways found to be activated (red) and inhibited (blue) among significant DEPs in WMLs. (D) Cell-specific expression of upregulated and downregulated proteins in WMLs. (E,F) Mean LFQ intensity-based heat map showing axonal (E) and oligodendrocytes (OL) specific proteins (F) in WMLs. (G-K) IHC images showing Di-amino benzidine (DAB) images of PLP (G) , MHCII (H) ERMN (I) , CNDP1 (J) , and ENPP2 (K) in the chronic WMLs from progressive MS brain. Sections (G,H) were counterstained with Haematoxylin. The inset shows immunofluorescence images of Olig2 co-localization with ERMN (I) , ENPP (J) , CNDP1 (K) . Scale bar: (G,H) , 200mum; (I-K) , 500 mum; inset, (I-K) , 10 mum.

PA5-12478

Antibody data