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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- GTX101343 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101343, RRID:AB_1240486
- Product name
- Ataxin 3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot analysis of ATXN3 expression in transfected 293T cell line by ATXN3 polyclonal antibody.A:Non-transfected lysate. B:ATXN3 transfected lysate.12% SDS PAGEGTX101343 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.293T mock transfected (-) or transfected with a construct expressing human Ataxin 3.10 % SDS-PAGEAtaxin 3 antibody (GTX101343) dilution: 1:20000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody (GTX101343) diluted by 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.Blue: Hoechst 33342 staining.
