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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50312PU-N - Provider product page
- Provider
- OriGene
- Product name
- Ataxin 3 (ATXN3) (Center) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Ataxin 3 (ATXN3) (Center) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATXN3
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ATXN3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). ATXN3 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ATXN3 (arrow) using rabbit polyclonal ATXN3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATXN3 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ATXN3 Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
