F55027

antibody from NSJ Bioreagents

Targeting: ATXN3 ATX3, JOS, MJD, SCA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55027

Provider

NSJ Bioreagents

Product name

Ataxin 3 Antibody / ATXN3

Antibody type

Polyclonal

Description

This highly specific Ataxin 3 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Ataxin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ataxin 3 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MCF7 cell lysate with Ataxin 3 antibody. Predicted molecular weight ~42 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with Ataxin 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human ZR-75-1 cells with Ataxin 3 antibody; Blue=isotype control, Green= Ataxin 3 antibody.