MA3-16515

antibody from Invitrogen Antibodies

Targeting: AIP ARA9, FKBP16, FKBP37, XAP2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA3-16515

Provider

Invitrogen Antibodies

Product name

AIP Monoclonal Antibody (35-2)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is specific for the FKBP domain. Suggested positive control: antigen standard for AIP (transient overexpression lysate).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

35-2

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Mechanistic insights into cancer cell killing through interaction of phosphodiesterase 3A and schlafen family member 12.

Wu X, Schnitzler GR, Gao GF, Diamond B, Baker AR, Kaplan B, Williamson K, Westlake L, Lorrey S, Lewis TA, Garvie CW, Lange M, Hayat S, Seidel H, Doench J, Cherniack AD, Kopitz C, Meyerson M, Greulich H
The Journal of biological chemistry 2020 Mar 13;295(11):3431-3446

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot detection of Ara9 in mouse lung lysates (30 µg) using Product # MA3-16515 at 1:500 with a 1 second exposure.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB detection on HEK 293T/17 cells. Lane 1: IP ARA9, WB ARA9, Lane 2: WB ARA9 with whole cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AIP in 0.5 mg/mL Hek293 lysate. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AIP in 0.5 mg/mL Hek293 lysate. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AIP in immersion fixed paraffin-embedded sections of human spleen. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in splenocytes.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of AIP in 1 x 10^6 CHO (A) and MCF-7 (B) cells. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.