Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22128 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Molt-4, Raji, mouse brain, PC-12, Rat2. Predicted reactivity: Mouse (94%), Rat (93%), Chicken (80%), Rhesus Monkey (97%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AIP Polyclonal Antibody detects ARA9 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg Molt-4 whole cell lysate/extract. C. 30 µg Raji whole cell lysate/extract.10 % SDS-PAGE. AIP Polyclonal Antibody (Product # PA5-22128) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AIP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AIP Polyclonal Antibody (Product # PA5-22128) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AIP Polyclonal Antibody (Product # PA5-22128). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. AIP Polyclonal Antibody (Product # PA5-22128) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AIP Polyclonal Antibody detects ARA9 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg Molt-4 whole cell lysate/extract. C. 30 µg Raji whole cell lysate/extract.10 % SDS-PAGE. AIP Polyclonal Antibody (Product # PA5-22128) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XAP2 in paraformaldehyde-fixed HeLa cells using a XAP2 polyclonal antibody (Product # PA5-22128) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARA9 antibody (Product # PA5-22128) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AIP Polyclonal Antibody detects ARA9 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. AIP Polyclonal Antibody (Product # PA5-22128) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.