19858-1-AP

antibody from Invitrogen Antibodies

Targeting: SRM SPS1, SRML1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

19858-1-AP

Provider

Invitrogen Antibodies

Product name

Spermidine synthase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEPGPDGPA ASGPAAIREG WFRETCSLWP GQALSLQVEQ LLHHRRSRYQ DILVFRSKTY GNVLVLDGVI QCTERDEFSY QEMIANLPLC SHPNPRKVLI IGGGDGGVLR EVVKHPSVES VVQCEIDEDV IQVSKKFLPG MAIGYSSSKL TLHVGDGFEF MKQNQDAFDV IITDSSDPMG PAESLFKESY YQLMKTALKE DGVLCCQGEC QWLHLDLIKE MRQFCQSLFP VVAYAYCTIP TYPSGQIGFM LCSKNPSTNF QEPVQPLTQQ QVAQMQLKYY NSDVHRAAFV LPEFARKALN DVS (1-302 aa encoded by BC000309)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LNCaP cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 19858-1-AP ( Spermidine synthase antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 19858-1-AP ( Spermidine synthase antibody at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Spermidine synthase (IP:19858-1-AP, 4ug; Detection:19858-1-AP 1:500) with mouse liver tissue lysate 4000ug.