MA5-50701

antibody from Invitrogen Antibodies

Targeting: UPK3A UPK3

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50701

Provider

Invitrogen Antibodies

Product name

UPK3A Recombinant Rabbit Monoclonal Antibody (PD00-09)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive control: Human bladder carcinoma tissue, Hela, 293. Predicted band size: 31 kDa Subcellular Location: Endoplasmic reticulum membrane.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

PD00-09

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of UPK3A in paraffin-embedded human bladder carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with UPK3A recombinant monoclonal antibody (Product # MA5-50701) with a dilution of 1:5,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of UPK3A in 293 cells. The cells were fixed, permeabilized, and then stained with UPK3A recombinant monoclonal antibody (Product # MA5-50701) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of UPK3A in Hela cells. The cells were fixed, permeabilized, and then stained with UPK3A recombinant monoclonal antibody (Product # MA5-50701) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).