Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-50701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UPK3A Recombinant Rabbit Monoclonal Antibody (PD00-09)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive control: Human bladder carcinoma tissue, Hela, 293. Predicted band size: 31 kDa Subcellular Location: Endoplasmic reticulum membrane.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- PD00-09
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of UPK3A in paraffin-embedded human bladder carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with UPK3A recombinant monoclonal antibody (Product # MA5-50701) with a dilution of 1:5,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of UPK3A in 293 cells. The cells were fixed, permeabilized, and then stained with UPK3A recombinant monoclonal antibody (Product # MA5-50701) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of UPK3A in Hela cells. The cells were fixed, permeabilized, and then stained with UPK3A recombinant monoclonal antibody (Product # MA5-50701) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).