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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-31220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX39 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Rat (98%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.
Soboleva TA, Parker BJ, Nekrasov M, Hart-Smith G, Tay YJ, Tng WQ, Wilkins M, Ryan D, Tremethick DJ
PLoS genetics 2017 Feb;13(2):e1006633
PLoS genetics 2017 Feb;13(2):e1006633
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX39 Polyclonal Antibody (Product # PA5-31220). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. DDX39 Polyclonal Antibody (Product # PA5-31220) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DDX39 was performed in paraffin-embedded human lung cancer tissue using DDX39 Polyclonal Antibody (Product # PA5-31220) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DDX39 was performed in 293T whole cell extracts using 5 µg of DDX39 Polyclonal Antibody (Product # PA5-31220). Samples were transferred to a membrane and probed with DDX39 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 4 H2A.B.3 co-immunoprecipitates with proteins involved in RNA processing and transcription. Mononucleosome-enriched chromatin from 28-30 day old mouse testes was immunoprecipitated with anti-H2A.B.3 or anti-H2A.Z antibodies. Co-immunoprecipitated proteins were identified by subsequent western blotting with the indicated antibodies selected to detect proteins involved in different aspects of RNA synthesis, processing and export. The presence of H2A.B.3, H2A.Z, histone H3 and H3K36me3 in the H2A.B.3 or H2A.Z co-immunoprecipitate were also examined by western blotting. Input chromatin (1/20 of the amount used for a ChIP-Seq experiment) was used as a loading control.
