Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-32109 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32109, RRID:AB_2153598
- Product name
- Rabbit Polyclonal NNT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NNT Antibody [NBP1-32109] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug HeLa whole cell lysate/extract, C. 30 ug HepG2 whole cell lysate/extract |. D. 30 ug A37C whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse stomach dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse kidney dilution: 1:500.