Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-27834 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NNT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-27834 targets NNT in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-27834 immunogen is a recombinant fragment corresponding to a region within amino acids 841 and 1113 of NNT. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.86 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NNT using A) 30 µg 293T whole cell lysate (B) 30 µg HeLa whole cell lysate and C) 30 µg HepG2 whole cell lysate. Samples were probed with a NNT polyclonal antibody (Product # PA5-27834).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNT in paraffin-embedded SW480 xenograft using a NNT polyclonal antibody (Product # PA5-27834) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNT in the cytosol of paraffin-embedded mouse stomach tissue using a NNT polyclonal antibody (Product # PA5-27834) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNT in the cytosol of paraffin-embedded mouse kidney using a NNT polyclonal antibody (Product # PA5-27834) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NNT in methanol-fixed A431 cells using a NNT polyclonal antibody (Product # PA5-27834) at a 1:200 dilution.