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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00004435-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004435-M01, RRID:AB_537782
- Product name
- Mouse Monoclonal CITED1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CITED1 - Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CITED1 Antibody (6G8) [H00004435-M01] - Analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ( Cat # H00004435-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody (M01), clone 6G8 (Cat # H00004435-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CITED1 Antibody (6G8) [H00004435-M01] - Analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody (M01), clone 6G8.Lane 1: CITED1 transfected lysate(19.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CITED1 Antibody (6G8) [H00004435-M01] - CITED1 monoclonal antibody (M01), clone 6G8 Analysis of CITED1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CITED1 Antibody (6G8) [H00004435-M01] - Detection limit for recombinant GST tagged CITED1 is approximately 0.3ng/ml as a capture antibody.
