PA5-34843

antibody from Invitrogen Antibodies

Targeting: DDX23 prp28, PRPF28, SNRNP100, U5-100K

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34843

Provider

Invitrogen Antibodies

Product name

DDX23 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, NIH-3T3, JC. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (86%), Rhesus Monkey (100%), Bovine (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.

Klimešová K, Vojáčková J, Radivojević N, Vandermoere F, Bertrand E, Verheggen C, Staněk D
Nature communications 2021 Jun 15;12(1):3646

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DDX23 in paraformaldehyde-fixed HeLa cells using a DDX23 polyclonal antibody (Product # PA5-34843) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 TSSC4 associates specifically with U5 snRNP and PRPF19 protein. a Characterization of proteins co-precipitating with TSSC4-GFP and TSSC4-FLAG by SILAC-IP. Primarily U5-specific proteins (yellow), Sm proteins (green), chaperones involved in U5 biogenesis (blue) co-purified with TSSC4. The ratio M/L shows a SILAC ratio of medium labeled proteins co-precipitated with tagged TSSC4 (M) and control immunoprecipitation from unlabeled cells (L). The experiment was performed once with TSSC4-GFP and once with TSSC4-FLAG. b TSSC4 interacts with proteins specific for U5 snRNP and the PRPF19 complex. TSSC4-GFP immunoprecipitation followed by western blotting and detection of proteins specific for U5 (PRPF8, SNRNP200, EFTUD2, and PRPF6--top panel and DDX23 and TXNL4A--bottom panel), U2 (SNRPA1) and U4/U6 (PRPF31 and SART3) snRNPs and PRPF19 complex (PRPF19 and CDC5L). SNRPB is one of the Sm proteins common to all snRNPs, and AAR2 is a PRPF8 chaperone. The positions of molecular weight markers are shown, the marker labeled by asterisk applies only to the GFP sample in the bottom combined GFP panels. See also Supplementary Fig. 1 for TSSC4-FLAG immunoprecipitation. c TSSC4 precipitates specifically U5 snRNA. TSSC4-GFP immunoprecipitation followed by RNA isolation and detection of co-purified RNAs by silver staining (top panel) and Northern blotting with a probe against U5 snRNA (bottom panel). Immunoprecipitation of Sm proteins with Y12 antibody served as a positive control and GFP as