Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-695A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse DDX23 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX23 antibody BL3135 (Cat. No. A300-695A) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). DDX23 was also immunoprecipitated by rabbit anti-DDX23 antibody BL3132 (Cat. No. A300-693A) but not by BL3134 (Cat. No. A300-694A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
