Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, NIH-3T3, JC. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX23 Polyclonal Antibody (Product # PA5-30312). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC . 7.5% SDS PAGE. DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX23 Polyclonal Antibody (Product # PA5-30312). Sample (30 µg of whole cell lysate). Lane A: JurKat . 7.5% SDS PAGE. DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX23 Polyclonal Antibody detects DDX23 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX23 protein stained by DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using DDX23 (Product # PA5-30312) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.