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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- R36394 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LFA-1 Antibody / CD11a
- Antibody type
- Polyclonal
- Description
- This highly specific LFA-1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the LFA-1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human spleen lysate with LFA-1 antibody at 0.5ug/ml. Predicted molecular weight ~129kDa, routinely observed at 180~200kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human spleen lysate with LFA-1 antibody at 0.5ug/ml. Predicted molecular weight: ~129 kDa (unmodified), up to 200 kDa (glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen with LFA-1 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen with LFA-1 antibody at 3.75ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lymph node with (top) and without (bottom) LFA-1 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen with LFA-1 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen with LFA-1 antibody at 3.75ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lymph node with (top) and without (bottom) LFA-1 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human ThP-1 cells with LFA-1 antibody (blue) at 10ug/ml and naive goat Ig (black).
