Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6942 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6942, RRID:AB_10680302
- Product name
- ITGAL monoclonal antibody, clone 38
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native ITGAL.
- Isotype
- IgG
- Antibody clone number
- 38
- Storage
- Store at 4°C.
Submitted references Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.
Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits.
Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL
The Journal of biological chemistry 1995 Jan 13;270(2):877-84
The Journal of biological chemistry 1995 Jan 13;270(2):877-84
Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N
The Journal of cell biology 1994 Jul;126(2):529-37
The Journal of cell biology 1994 Jul;126(2):529-37
Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits.
Dransfield I, Hogg N
The EMBO journal 1989 Dec 1;8(12):3759-65
The EMBO journal 1989 Dec 1;8(12):3759-65
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 5 X 105 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of ITGAL monoclonal antibody, clone 38 (Cat # MAB6942) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
- Validation comment
- Flow Cytometry