20985-1-AP

antibody from Proteintech Group

Targeting: DEPTOR DEP.6, DEPDC6, FLJ12428

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20985-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20985-1-AP, RRID:AB_11182391

Product name

DEPDC6/Deptor antibody

Antibody type

Polyclonal

Description

DEPDC6/Deptor antibody (Cat. #20985-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Skeletal diseases caused by mutations in PTH1R show aberrant differentiation of skeletal progenitors due to dysregulation of DEPTOR.

Csukasi F, Bosakova M, Barta T, Martin JH, Arcedo J, Barad M, Rico-Llanos GA, Zieba J, Becerra J, Krejci P, Duran I, Krakow D
Frontiers in cell and developmental biology 2022;10:963389

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NUMB facilitates autophagy initiation through targeting SCF(β-TrCP2) complex.

Li H, Shu S, Zhou M, Chen Y, Xiao A, Ma Y, Zhu F, Hu Z, Nie J
Cell death and differentiation 2022 Jul;29(7):1409-1422

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LncRNA KB-1471A8.2 Overexpression Suppresses Cell Proliferation and Migration and Antagonizes the Paclitaxel Resistance of Ovarian Cancer Cells.

Zhang M, Liu S, Fu C, Wang X, Zhang M, Liu G, Dai C, Gong Z, Xu H, Fu Z, Xu P, Xu J, Jia X
Cancer biotherapy & radiopharmaceuticals 2019 Jun;34(5):316-324

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TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.

Zhai Y, Lin P, Feng Z, Lu H, Han Q, Chen J, Zhang Y, He Q, Nan G, Luo X, Wang B, Feng F, Liu F, Chen Z, Zhu P
Autophagy 2018;14(9):1629-1643

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The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.

Csukasi F, Duran I, Barad M, Barta T, Gudernova I, Trantirek L, Martin JH, Kuo CY, Woods J, Lee H, Cohn DH, Krejci P, Krakow D
Science translational medicine 2018 Sep 19;10(459)

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DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.

Chen S, Jia L, Zhang S, Zheng Y, Zhou Y
Stem cell research & therapy 2018 Jul 4;9(1):185

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p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.

Yu X, Jin D, Yu A, Sun J, Chen X, Yang Z
Gene 2016 Sep 1;589(1):12-19

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Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.

Cheng M, Hu S, Wang Z, Pei Y, Fan R, Liu X, Wang L, Zhou J, Zheng S, Zhang T, Lin Y, Zhang M, Tao R, Zhong J
Oncotarget 2016 Jun 14;7(24):35643-35654

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20985-1-AP(DEPDC6 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The DEPDC6 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human DEPDC6. This antibody recognizes human,mouse,rat antigen. The DEPDC6 antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

20985-1-AP.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 20985-1-AP(DEPDC6 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 20985-1-AP(DEPDC6 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue