Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-49674 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49674, RRID:AB_10011798
- Product name
- Rabbit Polyclonal DEPTOR/DEPDC6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease.
DEPTOR inhibits cell proliferation and confers sensitivity to dopamine agonist in pituitary adenoma.
Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.
Amino Acid- and Insulin-Induced Activation of mTORC1 in Neonatal Piglet Skeletal Muscle Involves Sestin2-GATOR2, Rag A/C-mTOR, and RHEB-mTOR Complex Formation.
Loss of hepatic DEPTOR alters the metabolic transition to fasting.
The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway.
PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639.
Mediobasal hypothalamic overexpression of DEPTOR protects against high-fat diet-induced obesity.
DEP domain-containing mTOR-interacting protein in the rat brain: distribution of expression and potential implication.
MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1.
SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma.
PI3K independent activation of mTORC1 as a target in lapatinib-resistant ERBB2+ breast cancer cells.
Kim D, Park G, Huuhtanen J, Lundgren S, Khajuria RK, Hurtado AM, Muñoz-Calleja C, Cardeñoso L, Gómez-García de Soria V, Chen-Liang TH, Eldfors S, Ellonen P, Hannula S, Kankainen M, Bruck O, Kreutzman A, Salmenniemi U, Lönnberg T, Jerez A, Itälä-Remes M, Myllymäki M, Keränen MAI, Mustjoki S
Nature communications 2020 May 7;11(1):2246
Nature communications 2020 May 7;11(1):2246
DEPTOR inhibits cell proliferation and confers sensitivity to dopamine agonist in pituitary adenoma.
Yao H, Tang H, Zhang Y, Zhang QF, Liu XY, Liu YT, Gu WT, Zheng YZ, Shang HB, Wang Y, Huang JY, Wei YX, Zhang X, Zhang J, Wu ZB
Cancer letters 2019 Sep 10;459:135-144
Cancer letters 2019 Sep 10;459:135-144
Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.
Baek MO, Ahn CB, Cho HJ, Choi JY, Son KH, Yoon MS
Scientific reports 2019 Oct 17;9(1):14910
Scientific reports 2019 Oct 17;9(1):14910
Amino Acid- and Insulin-Induced Activation of mTORC1 in Neonatal Piglet Skeletal Muscle Involves Sestin2-GATOR2, Rag A/C-mTOR, and RHEB-mTOR Complex Formation.
Suryawan A, Davis TA
The Journal of nutrition 2018 Jun 1;148(6):825-833
The Journal of nutrition 2018 Jun 1;148(6):825-833
Loss of hepatic DEPTOR alters the metabolic transition to fasting.
Caron A, Mouchiroud M, Gautier N, Labbé SM, Villot R, Turcotte L, Secco B, Lamoureux G, Shum M, Gélinas Y, Marette A, Richard D, Sabatini DM, Laplante M
Molecular metabolism 2017 May;6(5):447-458
Molecular metabolism 2017 May;6(5):447-458
The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway.
Carbonneau M, M Gagné L, Lalonde ME, Germain MA, Motorina A, Guiot MC, Secco B, Vincent EE, Tumber A, Hulea L, Bergeman J, Oppermann U, Jones RG, Laplante M, Topisirovic I, Petrecca K, Huot MÉ, Mallette FA
Nature communications 2016 Sep 14;7:12700
Nature communications 2016 Sep 14;7:12700
PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639.
Song HI, Yoon MS
Scientific reports 2016 Nov 22;6:36968
Scientific reports 2016 Nov 22;6:36968
Mediobasal hypothalamic overexpression of DEPTOR protects against high-fat diet-induced obesity.
Caron A, Labbé SM, Lanfray D, Blanchard PG, Villot R, Roy C, Sabatini DM, Richard D, Laplante M
Molecular metabolism 2016 Feb;5(2):102-112
Molecular metabolism 2016 Feb;5(2):102-112
DEP domain-containing mTOR-interacting protein in the rat brain: distribution of expression and potential implication.
Caron A, Baraboi ED, Laplante M, Richard D
The Journal of comparative neurology 2015 Jan 1;523(1):93-107
The Journal of comparative neurology 2015 Jan 1;523(1):93-107
MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1.
Brundage ME, Tandon P, Eaves DW, Williams JP, Miller SJ, Hennigan RH, Jegga A, Cripe TP, Ratner N
Oncogene 2014 Dec 4;33(49):5626-36
Oncogene 2014 Dec 4;33(49):5626-36
SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma.
Fernández-Sáiz V, Targosz BS, Lemeer S, Eichner R, Langer C, Bullinger L, Reiter C, Slotta-Huspenina J, Schroeder S, Knorn AM, Kurutz J, Peschel C, Pagano M, Kuster B, Bassermann F
Nature cell biology 2013 Jan;15(1):72-81
Nature cell biology 2013 Jan;15(1):72-81
PI3K independent activation of mTORC1 as a target in lapatinib-resistant ERBB2+ breast cancer cells.
Jegg AM, Ward TM, Iorns E, Hoe N, Zhou J, Liu X, Singh S, Landgraf R, Pegram MD
Breast cancer research and treatment 2012 Dec;136(3):683-92
Breast cancer research and treatment 2012 Dec;136(3):683-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Analysis of DEPDC6 in (A) human liver and (B) mouse liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-49674] - PA regulates adipogenic differentiation by displacing DEPTOR from mTORC1. 3T3-L1 preadipocytes were transfected with a PPRE luciferase reporter plasmid vector, induced to differentiate for 3 h, and then luciferase activity was measured. 3T3-L1 cells were differentiated in the absence or presence of 300 uM C8-PA. Cell lysates and immunoprecipitates against raptor were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36968), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Staining of DEPTOR in mouse spleen using DAB with a hematoxylin counterstain.