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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-45977 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEPDC6 Recombinant Rabbit Monoclonal Antibody (PH00-03)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Predicted band size: 46 kDa Positive Control: MCF-7 cell lysate, Daudi cell lysate, PC-3M cell lysate, N2A cell lysate, L6 cell lysate, mouse brain tissue lysate, human small intestine tissue. Sequence Similarities: 96.35%Mouse; 97.73% Rat. Tissue Specificity: Expression is negatively regulated by both mTORC1 and mTORC2 (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- PH00-03
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DEPDC6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DEPDC6 Recombinant Rabbit Monoclonal Antibody (PH00-03) (Product # MA5-45977) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), 1:2,000 dilution, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
