PA5-117982

antibody from Invitrogen Antibodies

Targeting: HDLBP HBP, VGL

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117982

Provider

Invitrogen Antibodies

Product name

HDLBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HDLBP Polyclonal Antibody (Product # PA5-117982) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 141 kDa. Observed band size: 160 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HDLBP Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with HDLBP Polyclonal Antibody (Product # PA5-117982) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HDLBP Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 141 kDa. Observed band size: 160 kDa.